I-Antibody engathathi hlangothi

Ukungathathi hlangothi kwe-Antibody Featured Image

Inkcazelo emfutshane:

I-SARS-CoV-2 yintsholongwane ye-RNA egqunyiweyo kwaye enye.I-1ts ye-genome ye-RNA ifaka i-protein ye-nonstructural replicase kunye neeprotheni ezininzi zesakhiwo, kuquka i-spike (s), imvulophu (E), i-membrane (M) kunye ne-nucleocapsid (N) iiprotheni.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

I-Pathogenesis:

Iprotheyini ye-S inoxanduva lokubopha intsholongwane kunye nokungena kwiiseli ze-host, ezibandakanya ii-subunits ezimbini ezisebenzayo, i-s1 kunye ne-s2 kunye ne-receptor binding domain (RBD) ibekwe ngaphakathi kwee-subunits ze-s1. I-RBD ye-SARS-CoV-2 S protein isebenzisana kunye ne-angiotensin yokuguqula i-enzyme 2 (i-AcE2), ivuselela utshintsho oluhambelanayo kwiyunithi ye-s2 ephumela

kumxube wentsholongwane kunye nokungena kwiseli ekujoliswe kuyo.Iiproteases ezifihlakeleyo zabantu, ezifana ne-TMPRss2 kunye ne-furin, zifumaneka kwi-virally.target cells.

Ezi proteases ziphucula ukungena kwentsholongwane kwiiseli zomkhosi ngokusebenzisa iproteolysis yazo zombini iiproteni ze-s1, s2, kunye ne-AcE2.

Ukusetyenziswa okulindelekileyo:

I-Anti SARS-CoV-2 Neutralizing Antibody Rapid Test Kit yenzelwe ukufunyaniswa komgangatho we-SARS-CoV-2 wokungathathi hlangothi kwiisampulu zegazi lomntu.I-SARS-CoV-2 I-antibody engathathi hlangothi luphawu olubalulekileyo lokuvavanya ukusebenza kogonyo lwe-SARS-CoV-2.I-reagent yeyokuthomalalisa ukubhaqwa kwe-antibody kwiisampulu ezisuka ebantwini emva kokutofa isitofu okanye ukuchacha kwi-coV1D.19.Le khithi iya kunceda kuphando lwangoku lwe-coV1D.19 lokuxhaphaka kwe-sero, uvavanyo lokungakhuseleki komhlambi, ixesha elide lokhuselo lomzimba, ukusebenza kakuhle kwabagqatswa bamayeza abohlukeneyo kunye nokulandela usulelo kwizilwanyana.

iimeko zokugcina kunye nokuQinisekisa:

Zonke ii-reagents zilungele ukusetyenziswa njengoko zinikezelwe.Iikhithi ze-reagente ezingavulwanga zizinzile ku-4"c ～30"c kangangeenyanga ezingama-24 ngokwethutyana.I-1t kufuneka isetyenziswe kwiyure enye emva kokuba i-pouch ivuliwe.Musa ukuyikhenkcisa ikiti okanye uveze ikhithi ngaphezulu kwe-37"c ngexesha lokuyigcina.

Inkcazo:

1 uvavanyo / ibhokisi;Iimvavanyo ezi-5 / ibhokisi;Iimvavanyo ezingama-25 / ibhokisi;Iimvavanyo ezingama-50 / ibhokisi.

Inkqubo yoVavanyo:

Musa ukuvula isingxobo de ube ukulungele ukwenza uvavanyo, kwaye uvavanyo olulodwa.use lucetyiswa ukuba lusetyenziswe phantsi kokufuma okusingqongileyo okuphantsi (RHs70%） kwiyure enye.

1.Vumela onke amacandelo ekhithi kunye neesampulu ukuba zifikelele kwiqondo lobushushu begumbi phakathi kwe-18"c～26"c phambi kovavanyo.2.Susa ikhadi lovavanyo kwisingxobo sefoil kwaye ulibeke kwindawo ecocekileyo eyomileyo.

3.1chonga ikhadi lovavanyo lomzekelo ngamnye.

4.Sebenzisa i-dropper ukuhambisa enye i-drop (1) ye-serum, i-plasma okanye iisampulu zegazi elipheleleyo (40uL) kwisampulu kakuhle kwikhadi lokuvavanya, ilandelwa yi-drop of sample bufer.

5.qalisa isibali-xesha kwaye ufunde iziphumo kwimizuzu eyi-15.

Ukutolikwa kwesiphumo soVavanyo:

1tolika iziphumo zovavanyo ngokwale tshathi yombala ilandelayo (Njengokuba Ngezantsi).

1.1f ubukhulu bombala bungaphantsi kune-G4, ebonisa ukuba ugxininiso lwe-antibody engathathi hlangothi inkulu kuno-200 PRNT50 2.1f ubukhulu bombala buphakathi kwe-G4 kunye ne-G6, ebonisa ukuba ukuxinwa kwe-antibody engathathi hlangothi kujikeleze i-100 PRNT50 3.1f ubukhulu bombala bukufutshane ne-G7 , ebonisa ukuba ugxininiso kukuba i-antibody ingathathi hlangothi yi-50 PRNT50

4.Umda wokufumanisa yi-50 PRNT50

5.f ubukhulu bombala bunamandla kune-G7, bonisa isiphumo esibi.

Ngaphambili: I-Automatic Digital Wrist Monitor Blood Pressure

Okulandelayo: Incwadana-PocRoc®SARS-CoV-2 Antigen Rapid Test K0429