I-Serosurveillance ijongene nokuqikelela ukuxhaphaka kwezilwa-buhlungu kubemi ngokuchasene nentsholongwane ethile.Inceda ukulinganisa ukhuseleko lwabantu emva kosulelo okanye ugonyo kwaye ine-epidemiological utility ekulinganiseni umngcipheko wosulelo kunye namanqanaba okhuseleko lwabantu.Kubhubhani wesifo se-coronavirus sika-2019 (COVID-19), i-serosurvey idlale indima ebalulekileyo ekuvavanyeni inqanaba losulelo oluqatha lwe-coronavirus 2 (SARS-CoV-2) kubantu abahlukeneyo.Kukwancede ukuseka izikhombisi ze-epidemiologic, umz., umlinganiselo wokufa kosulelo (IFR).
Ekupheleni kuka-2020, iiserosurveys ezingama-400 zazipapashiwe.Ezi zifundo zazisekwe kwiintlobo ezahlukeneyo zovavanyo lwe-immunoassays olwaluyilelwe ukuhlalutya amajoni omzimba ngokuchasene ne-SARS-CoV-2, ngokuyintloko ejolise kuyo yonke okanye inxalenye ye-spike (S) kunye ne-nucleocapsid (N) yeeproteni ze-SARS-CoV-2.Kwimeko yangoku yobhubhane we-COVID-19, amaza obhubhani alandelelanayo ebesenzeka kwimimandla eyahlukeneyo yehlabathi, esosulela umxube wabantu abahlukeneyo ngexesha elithile.Lo mcimbi ucele umngeni kwi-SARS-CoV-2 serosurveillance ngenxa yokwanda kobume be-immunological landscape.
Izazinzulu ziye zaqaphela ukuba amanqanaba e-anti-SARS-CoV-2 anotyekelo lokubola emva kwexesha lokubuyela.Isiganeko esinjalo sandisa amathuba okuba neziphumo ezibi ngovavanyo lwe-immunoassays.Ezi zithintelo zingeyonyani zinokujongela phantsi ubuzaza belona zinga losulelo ngaphandle kokuba ziqatshelwe kwaye zilungiswe ngokukhawuleza.Ukongeza, i-antibody kinetics yasemva kokosuleleka ibonakala ngokwahlukileyo ngokuhambelana nobungqongqo bosulelo-usulelo oluqatha ngakumbi lwe-COVID-19 ludla ngokubandakanya ukonyuka okukhulu kwinqanaba le-antibodies xa kuthelekiswa nolosulelo oluthambileyo okanye olungenazimpawu.
Uphononongo oluninzi lubonakalise i-antibody kinetics kwiinyanga ezintandathu emva kosulelo.Olu phononongo lufumanise ukuba uninzi lwabantu ekuhlaleni abosulelwe yi-SARS-CoV-2 babonise usulelo oluthambileyo okanye olungenazimpawu.Abaphandi bakholelwa ukuba kubalulekile ukulinganisa utshintsho kumanqanaba e-antibodies, kusetyenziswa ii-immunoassays ezikhoyo, kuluhlu olubanzi lobunzima bosulelo.Ubudala bukwajongwe njengento ebalulekileyo kwezi zifundo.
Kuphononongo lwakutsha nje, izazinzulu ziye zalinganisa amanqanaba e-anti-SARS-CoV-2 antibody ukuya kuthi ga kwiinyanga ezili-9 emva kosulelo, kwaye bapapashe iziphumo zabomedRxiv* iseva yokuprinta kwangaphambili.Kuphononongo lwangoku, iqela labantu abane-seropositive baqeshwa ngovavanyo olwenziwe eGeneva, eSwitzerland.Abaphandi basebenzise i-immunoassays ezintathu ezahlukeneyo, ezizezi, i-semiquantitative anti-S1 ELISA yokufumanisa i-IgG (ebizwa ngokuba yi-EI), i-quantitative Elecsys anti-RBD (ebizwa ngokuba, i-Roche-S) kunye ne-semiquantitative Elecsys anti-N (ebizwa ngokuba yi-Roche- N).Uphando lwangoku lubonelela ngolwazi olubalulekileyo kwizifundo ezisekelwe kuluntu olusekwe kwi-serologic kwaye lubonisa ukuntsonkotha kwemeko yokhuselo lomzimba ngenxa yomxube wosulelo lwamva nje kunye nolukude lwe-COVID-19, kunye nogonyo.
Uphononongo oluphantsi koqwalaselo luchaze ukuba abantu abosulelwe yi-COVID-19 abaneempawu ezithambileyo okanye abane-asymptomatic, baveze ubukho be-antibodies.Ezi zithinteli-gazi zijolise mhlawumbi kwi-nucleocapsid (N) okanye kwi-spike (S) iiproteni ze-SARS-CoV-2 kwaye zafunyaniswa zizingisa ubuncinane iinyanga ezisi-8 emva kosulelo.Nangona kunjalo, ukufumanisa kwabo kuxhomekeke kakhulu ekukhethweni kwe-immunoassay.Abaphandi bafumanise ukuba imilinganiselo yokuqala ye-antibodies, ethatyathwe kubathathi-nxaxheba kwisithuba seenyanga ezine ezinesiqingatha se-COVID-19, yayingaguquguquki kuzo zonke iintlobo ezintathu zovavanyo lokuzikhusela olusetyenziswe kolu phononongo.Nangona kunjalo, emva kweenyanga ezine zokuqala, ukuya kuthi ga kwiinyanga ezisibhozo emva kosulelo, iziphumo zahlukana kulo lonke uvavanyo.
Olu phando lubonise ukuba kwimeko yovavanyo lwe-EI IgG, omnye kwabane abathathi-nxaxheba babene-sero-reverted.Nangona kunjalo, kwezinye ii-immunoassays, njenge-Roche anti-N kunye ne-anti-RBD iyonke yovavanyo lwe-Ig, kuphela ezimbalwa okanye akukho zi-sero-reversions zifunyenwe kwisampuli efanayo.Nabathathi-nxaxheba abanosulelo olungephi, ababecingelwa ngaphambili ukuba bafumane iimpendulo zokhuselo lomzimba olungamandla, babonakalise ubuntununtunu ngelixa besebenzisa i-anti-RBD kunye ne-anti-N iyonke yovavanyo lwe-Ig Roche.Zombini ezi mvavanyo zahlala zinobuntununtunu ngaphezulu kweenyanga ze-8 emva kosulelo.Ke, ezi ziphumo zityhila ukuba zombini i-Roche immunoassays ifaneleke ngakumbi ukuqikelela i-seroprevalence emva kwexesha elide emva kosulelo lokuqala.
Emva koko, usebenzisa uhlalutyo lokulinganisa, abaphandi bagqiba ekubeni ngaphandle kwendlela echanekileyo yokulinganisa, ngokukodwa, ngokuqwalasela uvakalelo lokuvavanywa kwexesha, uphando lwe-seroprevalence aluyi kuchaneka.Oku kuya kukhokelela ekujongeni phantsi kwelona nani losulelo olongezelekayo kubemi.Olu phononongo lwe-immunoassay lubonise ubukho bomahluko kumazinga e-seropositivity phakathi kweemvavanyo ezifumanekayo kurhwebo.
Kufuneka kuqatshelwe ukuba kukho imida emininzi kolu phononongo.Ngokomzekelo, i-reagent esetyenziswe ngelixa uqhuba uvavanyo lwe-EI kuzo zombini isiseko (uvavanyo lokuqala okanye lwe-1st) kunye nokulandelela (uvavanyo lwe-2 kubaviwa abafanayo) iisampuli ngaphakathi kwexesha elithile lahlukile.Omnye umda wolu phononongo kukuba amaqela awabandakanyi abantwana.Ukuza kuthi ga ngoku, akukho bungqina bexesha elide le-antibody dynamics ebantwaneni libhaliwe.
Ixesha lokuposa: Mar-24-2021